119151

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4913660..4914302 Replicon chromosome
Accession NZ_CP036004
Organism Bacillus mycoides strain BPN37/2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW61_RS25295 Protein ID WP_000635965.1
Coordinates 4913660..4914010 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW61_RS25300 Protein ID WP_000004570.1
Coordinates 4914015..4914302 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW61_RS25280 (EXW61_25760) 4910577..4911032 - 456 WP_016095131.1 SprT family protein -
EXW61_RS25285 (EXW61_25765) 4911243..4911359 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW61_RS25290 (EXW61_25770) 4911417..4913591 - 2175 WP_215599690.1 Tex family protein -
EXW61_RS25295 (EXW61_25775) 4913660..4914010 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW61_RS25300 (EXW61_25780) 4914015..4914302 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW61_RS25305 (EXW61_25785) 4914614..4915783 - 1170 WP_098168589.1 alanine racemase -
EXW61_RS25310 (EXW61_25790) 4915901..4916851 - 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
EXW61_RS25315 (EXW61_25795) 4917008..4917367 - 360 WP_002009969.1 holo-ACP synthase -
EXW61_RS25320 (EXW61_25800) 4917459..4918031 + 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW61_RS25325 (EXW61_25805) 4918024..4918986 - 963 WP_063218184.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119151 WP_000635965.1 NZ_CP036004:c4914010-4913660 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119151 NZ_CP036004:c4914010-4913660 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119151 WP_000004570.1 NZ_CP036004:c4914302-4914015 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119151 NZ_CP036004:c4914302-4914015 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References