Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2692976..2693618 Replicon chromosome
Accession NZ_CP035999
Organism Bacillus mycoides strain BPN37/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW27_RS13640 Protein ID WP_000635965.1
Coordinates 2692976..2693326 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW27_RS13645 Protein ID WP_000004570.1
Coordinates 2693331..2693618 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW27_RS13625 (EXW27_13890) 2689899..2690354 - 456 WP_016095131.1 SprT family protein -
EXW27_RS13630 (EXW27_13895) 2690565..2690681 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW27_RS13635 (EXW27_13900) 2690739..2692907 - 2169 WP_002124824.1 Tex family protein -
EXW27_RS13640 (EXW27_13905) 2692976..2693326 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW27_RS13645 (EXW27_13910) 2693331..2693618 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW27_RS13650 (EXW27_13915) 2693930..2695099 - 1170 WP_002124821.1 alanine racemase -
EXW27_RS13655 (EXW27_13920) 2695217..2696167 - 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
EXW27_RS13660 (EXW27_13925) 2696324..2696683 - 360 WP_002009969.1 holo-ACP synthase -
EXW27_RS13665 (EXW27_13930) 2696776..2697348 + 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW27_RS13670 (EXW27_13935) 2697341..2698303 - 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119148 WP_000635965.1 NZ_CP035999:c2693326-2692976 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119148 NZ_CP035999:c2693326-2692976 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119148 WP_000004570.1 NZ_CP035999:c2693618-2693331 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119148 NZ_CP035999:c2693618-2693331 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References