Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 598000..598978 Replicon chromosome
Accession NZ_CP035999
Organism Bacillus mycoides strain BPN37/1

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag EXW27_RS02985 Protein ID WP_002017051.1
Coordinates 598238..598978 (-) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW27_RS02980 Protein ID WP_000588712.1
Coordinates 598000..598125 (-) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW27_RS02955 (EXW27_03020) 593736..594395 - 660 WP_078179313.1 CatB-related O-acetyltransferase -
EXW27_RS02960 595108..595257 - 150 WP_002069316.1 hypothetical protein -
EXW27_RS02965 (EXW27_03025) 595617..597086 - 1470 WP_002127427.1 aminoacyl-histidine dipeptidase -
EXW27_RS02970 (EXW27_03030) 597338..597727 + 390 WP_078214330.1 YxeA family protein -
EXW27_RS02975 597748..597924 - 177 WP_003189253.1 stage II sporulation protein SB -
EXW27_RS02980 (EXW27_03035) 598000..598125 - 126 WP_000588712.1 hypothetical protein Antitoxin
EXW27_RS02985 (EXW27_03040) 598238..598978 - 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW27_RS02990 (EXW27_03045) 599318..600055 - 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW27_RS02995 (EXW27_03050) 600209..600685 - 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW27_RS03000 (EXW27_03055) 600959..601591 + 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
EXW27_RS03005 (EXW27_03060) 601768..602181 - 414 WP_002012925.1 VOC family protein -
EXW27_RS03010 (EXW27_03065) 602300..603010 - 711 WP_002127421.1 class I SAM-dependent methyltransferase -
EXW27_RS03015 (EXW27_03070) 603116..603877 - 762 WP_002127420.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T119147 WP_002017051.1 NZ_CP035999:c598978-598238 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119147 NZ_CP035999:c598978-598238 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119147 WP_000588712.1 NZ_CP035999:c598125-598000 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119147 NZ_CP035999:c598125-598000 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References