Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2535499..2536141 Replicon chromosome
Accession NZ_CP035997
Organism Bacillus mycoides strain BPN36/3

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW63_RS12685 Protein ID WP_000635965.1
Coordinates 2535499..2535849 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW63_RS12690 Protein ID WP_000004570.1
Coordinates 2535854..2536141 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW63_RS12670 (EXW63_12965) 2532422..2532877 - 456 WP_016095131.1 SprT family protein -
EXW63_RS12675 (EXW63_12970) 2533088..2533204 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW63_RS12680 (EXW63_12975) 2533262..2535430 - 2169 WP_215553124.1 Tex family protein -
EXW63_RS12685 (EXW63_12980) 2535499..2535849 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW63_RS12690 (EXW63_12985) 2535854..2536141 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW63_RS12695 (EXW63_12990) 2536453..2537622 - 1170 WP_061675821.1 alanine racemase -
EXW63_RS12700 (EXW63_12995) 2537740..2538690 - 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW63_RS12705 (EXW63_13000) 2538847..2539206 - 360 WP_002029438.1 holo-ACP synthase -
EXW63_RS12710 (EXW63_13005) 2539299..2539871 + 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW63_RS12715 (EXW63_13010) 2539864..2540826 - 963 WP_088038197.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119146 WP_000635965.1 NZ_CP035997:c2535849-2535499 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119146 NZ_CP035997:c2535849-2535499 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119146 WP_000004570.1 NZ_CP035997:c2536141-2535854 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119146 NZ_CP035997:c2536141-2535854 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTACCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References