Detailed information of TA system TA119144

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 333177..333987
• Replicon: plasmid p539
• Accession: NZ_CP035996
• Organism: Bacillus mycoides strain BPN36/2

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: EXW32_RS28815
• Protein ID: WP_215586239.1
• Coordinates: 333177..333590 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: EXW32_RS28820
• Protein ID: WP_215586240.1
• Coordinates: 333580..333987 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW32_RS28790 (EXW32_29460)	328723..329148	-	426	WP_002090470.1	DUF4879 domain-containing protein	-
EXW32_RS28795 (EXW32_29470)	329967..330176	-	210	WP_215586337.1	hypothetical protein	-
EXW32_RS28800 (EXW32_29475)	330582..330908	-	327	WP_215586237.1	metalloregulator ArsR/SmtB family transcription factor	-
EXW32_RS28805 (EXW32_29480)	331629..331979	+	351	WP_215586238.1	hypothetical protein	-
EXW32_RS28810 (EXW32_29485)	332325..332540	-	216	WP_001167047.1	alpha/beta-type small acid-soluble spore protein	-
EXW32_RS28815 (EXW32_29490)	333177..333590	-	414	WP_215586239.1	DUF86 domain-containing protein	Toxin
EXW32_RS28820 (EXW32_29495)	333580..333987	-	408	WP_215586240.1	nucleotidyltransferase domain-containing protein	Antitoxin
EXW32_RS28825 (EXW32_29505)	334598..335578	-	981	WP_215586241.1	hypothetical protein	-
EXW32_RS28830 (EXW32_29510)	335707..336450	-	744	WP_002068812.1	hypothetical protein	-
EXW32_RS28835 (EXW32_29515)	336450..337271	-	822	WP_215586242.1	DUF692 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..538903	538903

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (10-98)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15481.10 Da
• Isoelectric Point: 6.7144

>T119144 WP_215586239.1 NZ_CP035996:c333590-333177 [Bacillus mycoides]
MKNEVILNKISTIERCIKRIQDVYGNAPENLEDFTKQDSIILNIQRACEASIDLAMHIVAGKKLGLPQSSREAFDLLVTA
DLLSAELANKLKAMVGFRNIAVHDYQSVNLDIVRQIIEKHLKDFKLFTKEVMGILDL

Nucleotide

• Length: 414 bp

>T119144 NZ_CP035996:c333590-333177 [Bacillus mycoides]
ATGAAGAATGAGGTTATTTTAAATAAAATTTCTACAATAGAGCGTTGTATAAAAAGAATCCAAGATGTGTATGGAAATGC
CCCTGAAAATTTAGAGGATTTTACAAAACAAGATTCAATAATATTGAACATACAAAGAGCTTGTGAAGCGAGTATTGATT
TAGCAATGCATATTGTCGCAGGGAAAAAATTAGGATTACCTCAATCGAGTCGAGAGGCTTTTGATTTGCTGGTTACAGCA
GATTTACTTAGCGCAGAGCTAGCAAATAAGTTAAAGGCAATGGTTGGATTTCGAAATATCGCTGTTCATGATTATCAAAG
TGTAAATTTAGATATTGTTCGTCAAATTATTGAAAAACATCTAAAGGACTTTAAGCTGTTTACGAAAGAAGTTATGGGAA
TTCTGGACCTATAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15706.87 Da
• Isoelectric Point: 4.5000

>AT119144 WP_215586240.1 NZ_CP035996:c333987-333580 [Bacillus mycoides]
MIFQSMITAIIEKLQETVNPYWIIVFGSTAENREREDSDIDIAYLSDTTLGNYERFLLAQELANIVHRDVDLVDLSEAST
VFQAQIIHTGKTIFCSDDKRKVVFEMKTLKMYSKLNEERQVVFDDIKKRGSIYEE

Nucleotide

• Length: 408 bp

>AT119144 NZ_CP035996:c333987-333580 [Bacillus mycoides]
ATGATTTTTCAAAGCATGATAACAGCTATTATAGAAAAATTACAAGAGACGGTGAATCCCTATTGGATAATTGTGTTTGG
GTCAACGGCTGAGAATAGAGAACGAGAAGATAGTGATATTGATATAGCTTATTTAAGTGACACAACTTTAGGGAACTATG
AACGTTTTTTATTAGCACAAGAGTTAGCTAACATTGTTCATAGAGATGTTGATTTAGTTGATTTATCCGAAGCTTCTACA
GTTTTTCAAGCTCAGATTATACATACAGGAAAGACAATATTTTGTTCGGATGATAAACGTAAGGTCGTTTTTGAAATGAA
AACCTTAAAAATGTATTCAAAATTGAATGAAGAACGACAGGTAGTATTTGATGATATAAAGAAAAGAGGTTCAATATATG
AAGAATGA