Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2387183..2388161 Replicon chromosome
Accession NZ_CP035994
Organism Bacillus mycoides strain BPN36/2

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag EXW32_RS12105 Protein ID WP_002017051.1
Coordinates 2387183..2387923 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW32_RS12110 Protein ID WP_000588712.1
Coordinates 2388036..2388161 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW32_RS12075 (EXW32_12355) 2382285..2383046 + 762 WP_002127420.1 ABC transporter ATP-binding protein -
EXW32_RS12080 (EXW32_12360) 2383152..2383862 + 711 WP_002127421.1 class I SAM-dependent methyltransferase -
EXW32_RS12085 (EXW32_12365) 2383981..2384394 + 414 WP_002012925.1 VOC family protein -
EXW32_RS12090 (EXW32_12370) 2384571..2385203 - 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
EXW32_RS12095 (EXW32_12375) 2385477..2385953 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW32_RS12100 (EXW32_12380) 2386107..2386844 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW32_RS12105 (EXW32_12385) 2387183..2387923 + 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW32_RS12110 (EXW32_12390) 2388036..2388161 + 126 WP_000588712.1 hypothetical protein Antitoxin
EXW32_RS12115 2388237..2388413 + 177 WP_003189253.1 stage II sporulation protein SB -
EXW32_RS12120 (EXW32_12395) 2388434..2388823 - 390 WP_033733970.1 YxeA family protein -
EXW32_RS12125 (EXW32_12400) 2389075..2390544 + 1470 WP_016126945.1 aminoacyl-histidine dipeptidase -
EXW32_RS12130 2390904..2391053 + 150 WP_002069316.1 hypothetical protein -
EXW32_RS12135 (EXW32_12405) 2391767..2392426 + 660 WP_003189261.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T119143 WP_002017051.1 NZ_CP035994:2387183-2387923 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119143 NZ_CP035994:2387183-2387923 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119143 WP_000588712.1 NZ_CP035994:2388036-2388161 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119143 NZ_CP035994:2388036-2388161 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References