Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2279266..2279908 Replicon chromosome
Accession NZ_CP035987
Organism Bacillus mycoides strain BPN29/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW60_RS11850 Protein ID WP_000635965.1
Coordinates 2279558..2279908 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW60_RS11845 Protein ID WP_000004570.1
Coordinates 2279266..2279553 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW60_RS11820 (EXW60_12045) 2274582..2275544 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW60_RS11825 (EXW60_12050) 2275537..2276109 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW60_RS11830 (EXW60_12055) 2276201..2276560 + 360 WP_002009969.1 holo-ACP synthase -
EXW60_RS11835 (EXW60_12060) 2276717..2277667 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW60_RS11840 (EXW60_12065) 2277785..2278954 + 1170 WP_002009971.1 alanine racemase -
EXW60_RS11845 (EXW60_12070) 2279266..2279553 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW60_RS11850 (EXW60_12075) 2279558..2279908 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW60_RS11855 (EXW60_12080) 2279977..2282145 + 2169 WP_002029442.1 Tex family protein -
EXW60_RS11860 (EXW60_12085) 2282203..2282319 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW60_RS11865 (EXW60_12090) 2282530..2282985 + 456 WP_215587167.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119140 WP_000635965.1 NZ_CP035987:2279558-2279908 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119140 NZ_CP035987:2279558-2279908 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119140 WP_000004570.1 NZ_CP035987:2279266-2279553 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119140 NZ_CP035987:2279266-2279553 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References