Detailed information of TA system TA119122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5488863..5489514
• Replicon: chromosome
• Accession: NZ_CP035952
• Organism: Pseudomonas sp. SNU WT1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EXN22_RS25265
• Protein ID: WP_130266580.1
• Coordinates: 5489332..5489514 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXN22_RS25260
• Protein ID: WP_130266579.1
• Coordinates: 5488863..5489267 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXN22_RS25250	5484891..5488634	+	3744	WP_130266578.1	bifunctional diguanylate cyclase/phosphodiesterase	-
EXN22_RS25260	5488863..5489267	-	405	WP_130266579.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EXN22_RS25265	5489332..5489514	-	183	WP_130266580.1	type II toxin-antitoxin system HicA family toxin	Toxin
EXN22_RS25270	5489649..5490446	-	798	WP_130266581.1	helix-turn-helix transcriptional regulator	-
EXN22_RS25275	5490531..5491349	+	819	WP_130266582.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
EXN22_RS25280	5491442..5491903	+	462	WP_130266583.1	Lrp/AsnC family transcriptional regulator	-
EXN22_RS25285	5492022..5492360	+	339	WP_130266584.1	toxin	-
EXN22_RS25290	5492362..5492676	+	315	WP_130266585.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6685.72 Da
• Isoelectric Point: 11.2655

>T119122 WP_130266580.1 NZ_CP035952:c5489514-5489332 [Pseudomonas sp. SNU WT1]
MRSREIIALLEADGWQEVGVRGSHHHFKHPTRPGRVTVPHPKSDLPKGTLNNILKTAGLK

Nucleotide

• Length: 183 bp

>T119122 NZ_CP035952:c5489514-5489332 [Pseudomonas sp. SNU WT1]
ATGCGAAGTAGGGAGATTATCGCGTTACTTGAGGCGGATGGATGGCAAGAGGTCGGGGTGAGAGGTAGTCACCACCACTT
CAAGCATCCAACCAGACCCGGCAGGGTGACAGTCCCGCATCCCAAGAGCGATTTACCGAAAGGGACGCTGAACAACATAT
TGAAAACCGCTGGCCTCAAGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14642.41 Da
• Isoelectric Point: 4.2146

>AT119122 WP_130266579.1 NZ_CP035952:c5489267-5488863 [Pseudomonas sp. SNU WT1]
MKFPVVIHKDPDSDYGVIVPDVAGCFSSGTTVEEALDNVQEALALHFESLVADEEPLPQGQAIDVHIDNPDYAGGVWAVV
DFDVTPYLGKSVRFNASLPEFLLQRIDSYVKNHPEQKSRSGFLASAALRVLQEA

Nucleotide

• Length: 405 bp

>AT119122 NZ_CP035952:c5489267-5488863 [Pseudomonas sp. SNU WT1]
ATGAAATTTCCAGTCGTCATTCATAAAGACCCCGATTCAGACTATGGCGTGATTGTGCCGGATGTTGCGGGCTGCTTCTC
TTCAGGTACAACCGTCGAAGAAGCGCTGGACAATGTCCAGGAGGCTTTGGCCCTGCACTTCGAGTCACTGGTCGCCGACG
AGGAGCCACTGCCTCAGGGCCAGGCTATTGATGTCCATATCGATAATCCCGACTATGCGGGCGGTGTGTGGGCAGTCGTT
GATTTTGATGTGACGCCGTACTTGGGCAAGTCAGTGCGGTTCAATGCGTCTCTGCCGGAGTTCCTGCTGCAGCGCATCGA
TAGCTATGTAAAGAATCATCCCGAACAGAAAAGCCGATCGGGTTTTCTGGCATCAGCTGCGCTTCGAGTCTTACAGGAGG
CGTGA