Detailed information of TA system TA119117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 960804..961444
• Replicon: chromosome
• Accession: NZ_CP035952
• Organism: Pseudomonas sp. SNU WT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXN22_RS04370
• Protein ID: WP_130262918.1
• Coordinates: 960804..961214 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EXN22_RS04375
• Protein ID: WP_130262919.1
• Coordinates: 961214..961444 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXN22_RS04340	955834..957900	-	2067	WP_130262912.1	TonB-dependent copper receptor	-
EXN22_RS04345	957978..958400	-	423	WP_130262913.1	DUF2946 domain-containing protein	-
EXN22_RS04350	958420..958890	-	471	WP_130262914.1	copper chaperone PCu(A)C	-
EXN22_RS04355	958938..959330	-	393	WP_130262915.1	DUF2946 domain-containing protein	-
EXN22_RS04360	959427..960284	-	858	WP_130262916.1	SPFH/Band 7/PHB domain protein	-
EXN22_RS04365	960308..960745	-	438	WP_130262917.1	NfeD family protein	-
EXN22_RS04370	960804..961214	-	411	WP_130262918.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EXN22_RS04375	961214..961444	-	231	WP_130262919.1	antitoxin	Antitoxin
EXN22_RS04380	961504..962250	-	747	WP_130262920.1	cobalt-precorrin-6A reductase	-
EXN22_RS04385	962247..963344	-	1098	WP_130262921.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
EXN22_RS04390	963337..964548	-	1212	WP_130262922.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
EXN22_RS04395	964665..965999	+	1335	WP_130262923.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15117.46 Da
• Isoelectric Point: 7.3836

>T119117 WP_130262918.1 NZ_CP035952:c961214-960804 [Pseudomonas sp. SNU WT1]
MLKYMLDTNICIFTFKNKPQAVREAFNRHHGQLCISTVTLMELIYGAEKSAAPERNLAVVEGFAARLEVLSYDEHAAAHS
GQLRAELARAGTPIGPYDQMIAGHARSLGLLLVTNNMREFLRVPGLRVEDWLNPAP

Nucleotide

• Length: 411 bp

>T119117 NZ_CP035952:c961214-960804 [Pseudomonas sp. SNU WT1]
ATGCTCAAGTACATGCTTGATACCAACATTTGCATCTTTACCTTCAAGAACAAGCCTCAGGCCGTACGTGAGGCCTTCAA
TCGCCACCACGGGCAGCTTTGCATCAGCACAGTAACGCTGATGGAGCTGATTTATGGTGCAGAGAAGTCTGCCGCGCCTG
AACGTAACCTTGCGGTAGTCGAAGGTTTTGCCGCACGCCTGGAAGTCCTCAGTTACGATGAGCACGCCGCCGCTCATAGC
GGTCAGTTGCGTGCGGAACTGGCCAGAGCCGGAACACCGATTGGGCCCTATGACCAGATGATTGCCGGGCATGCACGGTC
TCTGGGGCTGTTACTGGTAACCAACAACATGCGTGAGTTTTTGCGTGTTCCGGGGCTGCGTGTGGAAGACTGGCTCAACC
CTGCGCCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8566.50 Da
• Isoelectric Point: 4.4367

>AT119117 WP_130262919.1 NZ_CP035952:c961444-961214 [Pseudomonas sp. SNU WT1]
MEQGAVFKSNRSQAIRLPKSVALPEDVKRVDIVAVGRTRIISPSGEAWDSWFEGQDVSPDFMAEREQNADQEREGF

Nucleotide

• Length: 231 bp

>AT119117 NZ_CP035952:c961444-961214 [Pseudomonas sp. SNU WT1]
ATGGAGCAAGGTGCCGTCTTCAAGAGCAACCGCAGCCAAGCTATACGGCTGCCCAAATCCGTAGCCCTGCCAGAGGATGT
GAAGCGCGTGGATATAGTCGCAGTGGGTCGAACCCGAATTATTTCCCCCTCCGGCGAAGCCTGGGACAGCTGGTTCGAAG
GGCAGGACGTCAGCCCGGATTTCATGGCCGAGCGCGAGCAGAATGCCGATCAGGAGCGCGAGGGCTTTTGA