Detailed information of TA system TA119101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1369305..1369930
• Replicon: chromosome
• Accession: NZ_CP035944
• Organism: Escherichia coli strain AR24.2b

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXT04_RS06740
• Protein ID: WP_000911330.1
• Coordinates: 1369532..1369930 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EXT04_RS06735
• Protein ID: WP_000450524.1
• Coordinates: 1369305..1369532 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXT04_RS06710	1365108..1365578	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EXT04_RS06715	1365578..1366150	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EXT04_RS06720	1366296..1367174	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXT04_RS06725	1367191..1368225	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EXT04_RS06730	1368438..1369151	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXT04_RS06735	1369305..1369532	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXT04_RS06740	1369532..1369930	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EXT04_RS06745	1370077..1370940	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
EXT04_RS06750	1370955..1372970	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXT04_RS06755	1373044..1373742	+	699	WP_000679823.1	esterase	-
EXT04_RS06760	1373852..1374052	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T119101 WP_000911330.1 NZ_CP035944:1369532-1369930 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T119101 NZ_CP035944:1369532-1369930 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT119101 WP_000450524.1 NZ_CP035944:1369305-1369532 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT119101 NZ_CP035944:1369305-1369532 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33