Detailed information of TA system TA119061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 71853..72490
• Replicon: plasmid plas2
• Accession: NZ_CP035903
• Organism: Burkholderia glumae strain 257sh-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: EXE55_RS28775
• Protein ID: WP_012735347.1
• Coordinates: 71853..72254 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EXE55_RS28780
• Protein ID: WP_017924531.1
• Coordinates: 72254..72490 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE55_RS28740	67065..67499	-	435	WP_161522145.1	helix-turn-helix transcriptional regulator	-
EXE55_RS28745	67609..68226	+	618	WP_161522128.1	NAD(P)H-dependent oxidoreductase	-
EXE55_RS28750	68231..68884	+	654	WP_161522129.1	SDR family oxidoreductase	-
EXE55_RS28755	68914..69825	+	912	WP_161522130.1	SDR family NAD(P)-dependent oxidoreductase	-
EXE55_RS28760	69849..70490	+	642	WP_161522131.1	DsbA family protein	-
EXE55_RS28765	70575..70697	+	123	Protein_69	AAA family ATPase	-
EXE55_RS28770	70896..71624	-	729	WP_012735342.1	IS6 family transposase	-
EXE55_RS28775	71853..72254	-	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXE55_RS28780	72254..72490	-	237	WP_017924531.1	AbrB family transcriptional regulator	Antitoxin
EXE55_RS28785	72627..73286	-	660	WP_161522132.1	tyrosine-type recombinase/integrase	-
EXE55_RS28790	73401..73691	-	291	WP_161522133.1	hypothetical protein	-
EXE55_RS29585	73731..73898	-	168	WP_174525173.1	hypothetical protein	-
EXE55_RS29590	74068..74238	-	171	WP_174525174.1	hypothetical protein	-
EXE55_RS28800	74309..74992	-	684	WP_161522143.1	IS6 family transposase	-
EXE55_RS28805	75086..75903	+	818	WP_085962346.1	IS5 family transposase	-
EXE55_RS28810	75999..76193	+	195	WP_124837685.1	hypothetical protein	-
EXE55_RS28815	76343..76564	+	222	WP_017433768.1	hypothetical protein	-
EXE55_RS28820	76787..77251	+	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..175525	175525
	-	flank	IS/Tn	-	-	75086..75496	410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T119061 WP_012735347.1 NZ_CP035903:c72254-71853 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T119061 NZ_CP035903:c72254-71853 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8965.10 Da
• Isoelectric Point: 6.3676

>AT119061 WP_017924531.1 NZ_CP035903:c72490-72254 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT119061 NZ_CP035903:c72490-72254 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTCTGTAA

Structures

Toxin

• AlphaFold DB: C5AP38