Detailed information of TA system TA119060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 43868..44405
• Replicon: plasmid plas1
• Accession: NZ_CP035902
• Organism: Burkholderia glumae strain 257sh-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: C5AN85
• Locus tag: EXE55_RS27535
• Protein ID: WP_012734112.1
• Coordinates: 43868..44170 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: C5AN86
• Locus tag: EXE55_RS27540
• Protein ID: WP_012734113.1
• Coordinates: 44160..44405 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE55_RS27490	40253..40594	-	342	WP_161522030.1	hypothetical protein	-
EXE55_RS27495	40591..40827	-	237	WP_161522031.1	hypothetical protein	-
EXE55_RS27500	40923..41162	-	240	WP_161522032.1	hypothetical protein	-
EXE55_RS27505	41175..41348	-	174	WP_161522033.1	hypothetical protein	-
EXE55_RS27510	41540..41755	-	216	WP_161522034.1	hypothetical protein	-
EXE55_RS27515	42017..42340	-	324	WP_043308435.1	hypothetical protein	-
EXE55_RS27520	42518..42799	-	282	WP_043308454.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EXE55_RS27525	42803..43258	-	456	WP_012734111.1	hypothetical protein	-
EXE55_RS27530	43466..43708	-	243	WP_043308436.1	hypothetical protein	-
EXE55_RS27535	43868..44170	-	303	WP_012734112.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXE55_RS27540	44160..44405	-	246	WP_012734113.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EXE55_RS27545	44559..44753	-	195	WP_043308437.1	hypothetical protein	-
EXE55_RS27550	46069..46740	+	672	WP_012734035.1	AAA family ATPase	-
EXE55_RS27555	46740..47705	+	966	WP_012734036.1	ParB/RepB/Spo0J family partition protein	-
EXE55_RS27560	48460..49317	+	858	WP_012734037.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..214469	214469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-92)

Antitoxin

• Antitoxin ParD (1-80)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11548.11 Da
• Isoelectric Point: 6.7171

>T119060 WP_012734112.1 NZ_CP035902:c44170-43868 [Burkholderia glumae]
MRGKARVVRLTPLAEADLEDIWAYIFEQWSLAQAEQYVGEIVAAFNRLARGEWVGAVRGEPYRRYLVGFHAVFYRETTDA
LDVACVRHQRMDVGSHLSQY

Nucleotide

• Length: 303 bp

>T119060 NZ_CP035902:c44170-43868 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8645.69 Da
• Isoelectric Point: 7.2029

>AT119060 WP_012734113.1 NZ_CP035902:c44405-44160 [Burkholderia glumae]
MPRNTSISLGDHLTQFVDTQVASGRYGSASDVVRAGLRLLETQETELKALKEALRASEASGAPAAFDGQAFLDRMRAKHA
G

Nucleotide

• Length: 246 bp

>AT119060 NZ_CP035902:c44405-44160 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Structures

Toxin

• AlphaFold DB: C5AN85

Antitoxin

• AlphaFold DB: C5AN86