Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1641329..1641997 Replicon chromosome
Accession NZ_CP035897
Organism Streptococcus pneumoniae strain EF3030

Toxin (Protein)


Gene name hicA Uniprot ID B1I7Q0
Locus tag EF3030_RS08535 Protein ID WP_001132285.1
Coordinates 1641818..1641997 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag EF3030_RS08530 Protein ID WP_000961810.1
Coordinates 1641329..1641781 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF3030_RS08510 1638178..1639212 - 1035 WP_000747915.1 ATP-binding protein -
EF3030_RS08515 1639322..1639480 - 159 WP_000401986.1 7,8-dihydro-8-oxoguanine-triphosphatase -
EF3030_RS08520 1639482..1640549 - 1068 WP_000719723.1 M50 family metallopeptidase -
EF3030_RS08525 1640568..1641038 - 471 WP_000257084.1 DUF3013 family protein -
EF3030_RS08530 1641329..1641781 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EF3030_RS08535 1641818..1641997 - 180 WP_001132285.1 type II toxin-antitoxin system HicA family toxin Toxin
EF3030_RS08540 1642134..1642313 - 180 WP_001048906.1 hypothetical protein -
EF3030_RS08550 1642478..1642717 - 240 WP_000818078.1 hypothetical protein -
EF3030_RS08560 1642987..1644258 + 1272 WP_001113218.1 replication-associated recombination protein A -
EF3030_RS08570 1644741..1646000 + 1260 Protein_1614 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1644705..1646000 1295


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.01 Da        Isoelectric Point: 11.2964

>T119058 WP_001132285.1 NZ_CP035897:c1641997-1641818 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGKLNKYTERGIRKQAGL

Download         Length: 180 bp

>T119058 NZ_CP035897:c1641997-1641818 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT119058 WP_000961810.1 NZ_CP035897:c1641781-1641329 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT119058 NZ_CP035897:c1641781-1641329 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0T9HEZ5

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References