Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 151221..151813 Replicon plasmid pLSN03016011A_163
Accession NZ_CP035885
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag EW995_RS25075 Protein ID WP_134737242.1
Coordinates 151221..151469 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EW995_RS25080 Protein ID WP_000681610.1
Coordinates 151466..151813 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS25045 (146507) 146507..146881 + 375 WP_024167121.1 heat-labile enterotoxin LT subunit B -
EW995_RS25050 (147203) 147203..147937 - 735 Protein_182 IS3-like element IS600 family transposase -
EW995_RS25055 (147973) 147973..149186 - 1214 Protein_183 IS3 family transposase -
EW995_RS25060 (149212) 149212..149967 - 756 WP_134737243.1 IS3 family transposase -
EW995_RS25065 (149943) 149943..150449 - 507 WP_074504062.1 helix-turn-helix domain-containing protein -
EW995_RS25070 (150502) 150502..150756 + 255 WP_167553975.1 hypothetical protein -
EW995_RS25075 (151221) 151221..151469 + 249 WP_134737242.1 type II toxin-antitoxin system HicA family toxin Toxin
EW995_RS25080 (151466) 151466..151813 + 348 WP_000681610.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW995_RS25085 (152040) 152040..153217 - 1178 Protein_189 IS91 family transposase -
EW995_RS25090 (153217) 153217..153582 - 366 WP_074480516.1 transposase -
EW995_RS25095 (153664) 153664..154272 + 609 Protein_191 DDE-type integrase/transposase/recombinase -
EW995_RS25100 (154475) 154475..156286 + 1812 WP_167553976.1 two-partner secretion system transporter EtpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - csbD / csbC / csbA / csbB / eltA / eltB / etpB / etpA 1..163137 163137
- inside IScluster/Tn - eltA / eltB 142483..154272 11789


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9321.96 Da        Isoelectric Point: 10.6270

>T119054 WP_134737242.1 NZ_CP035885:151221-151469 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAGINHTILMHRPHPESYIKGGTLKTIKQNLKEAG
VL

Download         Length: 249 bp

>T119054 NZ_CP035885:151221-151469 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATT
TTCTTCTCTAGGATATGTCAAAAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGGAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13152.97 Da        Isoelectric Point: 4.9966

>AT119054 WP_000681610.1 NZ_CP035885:151466-151813 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Download         Length: 348 bp

>AT119054 NZ_CP035885:151466-151813 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References