Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 127697..128340 Replicon plasmid pLSN03016011A_163
Accession NZ_CP035885
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name vagD Uniprot ID A0A4U9UKM6
Locus tag EW995_RS24940 Protein ID WP_001505096.1
Coordinates 127697..128113 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5E8P385
Locus tag EW995_RS24945 Protein ID WP_001505095.1
Coordinates 128110..128340 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS24925 (122886) 122886..123302 - 417 WP_024167721.1 type II toxin-antitoxin system VapC family toxin -
EW995_RS24930 (123299) 123299..123529 - 231 WP_024167720.1 type II toxin-antitoxin system VapB family antitoxin -
EW995_RS24935 (123858) 123858..127652 + 3795 WP_001505097.1 hypothetical protein -
EW995_RS24940 (127697) 127697..128113 - 417 WP_001505096.1 type II toxin-antitoxin system VapC family toxin Toxin
EW995_RS24945 (128110) 128110..128340 - 231 WP_001505095.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EW995_RS24950 (128927) 128927..129277 + 351 WP_000493398.1 hypothetical protein -
EW995_RS24955 (129321) 129321..129536 + 216 Protein_163 hypothetical protein -
EW995_RS24960 (129588) 129588..130292 - 705 WP_216349507.1 IS6-like element IS26 family transposase -
EW995_RS24965 (130283) 130283..130789 + 507 WP_001505085.1 hypothetical protein -
EW995_RS24970 (130786) 130786..131577 + 792 Protein_166 site-specific integrase -
EW995_RS24975 (131715) 131715..131990 - 276 WP_000239529.1 hypothetical protein -
EW995_RS24980 (131984) 131984..132628 - 645 WP_000633913.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - csbD / csbC / csbA / csbB / eltA / eltB / etpB / etpA 1..163137 163137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14966.31 Da        Isoelectric Point: 6.9683

>T119053 WP_001505096.1 NZ_CP035885:c128113-127697 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKHLEQAVLRGHRIVVSAITYAEMRFGATGPKASPRHVHLVDAFCARLDAILPWDRA
AADATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T119053 NZ_CP035885:c128113-127697 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGCATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTCGTCTCGGCCATCACCTACGCCGAGATGCGCTTCGGTGCCACCGGTC
CAAAGGCCTCGCCACGCCACGTGCATCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGCGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACACCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTAACGAATAATGTGAGGGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8836.02 Da        Isoelectric Point: 4.6310

>AT119053 WP_001505095.1 NZ_CP035885:c128340-128110 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDNIILRPVRPTWSSFLELEKADPDFMAKREDIVSDEGRFNL

Download         Length: 231 bp

>AT119053 NZ_CP035885:c128340-128110 [Escherichia coli]
ATGAGAACCGTATCCATTTTCAAAAACGGCAATAACCGCGCCATCCGCCTTCCCCGAGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATTGTCCGGGAAGGGGACAACATCATCCTGCGCCCTGTCCGGCCGACCTGGAGCTCATTCCTGGAGCTCG
AAAAGGCCGATCCGGACTTTATGGCGAAGCGTGAGGACATTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9UKM6

Antitoxin

Source ID Structure
AlphaFold DB A0A5E8P385

References