Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 122886..123529 Replicon plasmid pLSN03016011A_163
Accession NZ_CP035885
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name vagD Uniprot ID A0A4U9UJ30
Locus tag EW995_RS24925 Protein ID WP_024167721.1
Coordinates 122886..123302 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5E8P3V3
Locus tag EW995_RS24930 Protein ID WP_024167720.1
Coordinates 123299..123529 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS25340 (119175) 119175..119703 - 529 Protein_153 hypothetical protein -
EW995_RS24910 (120025) 120025..120198 + 174 WP_162173578.1 hypothetical protein -
EW995_RS24915 (120240) 120240..121262 - 1023 WP_001505100.1 hypothetical protein -
EW995_RS24920 (121247) 121247..122812 - 1566 WP_001505099.1 AAA family ATPase -
EW995_RS24925 (122886) 122886..123302 - 417 WP_024167721.1 type II toxin-antitoxin system VapC family toxin Toxin
EW995_RS24930 (123299) 123299..123529 - 231 WP_024167720.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EW995_RS24935 (123858) 123858..127652 + 3795 WP_001505097.1 hypothetical protein -
EW995_RS24940 (127697) 127697..128113 - 417 WP_001505096.1 type II toxin-antitoxin system VapC family toxin -
EW995_RS24945 (128110) 128110..128340 - 231 WP_001505095.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - csbD / csbC / csbA / csbB / eltA / eltB / etpB / etpA 1..163137 163137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15290.70 Da        Isoelectric Point: 6.4760

>T119052 WP_024167721.1 NZ_CP035885:c123302-122886 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFDATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T119052 NZ_CP035885:c123302-122886 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGATGCCACAGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8762.83 Da        Isoelectric Point: 4.3775

>AT119052 WP_024167720.1 NZ_CP035885:c123529-123299 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNQ

Download         Length: 231 bp

>AT119052 NZ_CP035885:c123529-123299 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGTGCCATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9UJ30

Antitoxin

Source ID Structure
AlphaFold DB A0A5E8P3V3

References