119042

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 4400268..4401988 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name pmenT Uniprot ID -
Locus tag EW995_RS20985 Protein ID WP_216349480.1
Coordinates 4401332..4401988 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag EW995_RS20980 Protein ID WP_000634202.1
Coordinates 4400268..4401335 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS20960 (4395401) 4395401..4396078 - 678 WP_001339397.1 IS66-like element accessory protein TnpA -
EW995_RS20965 (4396122) 4396122..4396274 + 153 Protein_4089 IS110 family transposase -
EW995_RS20970 (4396438) 4396438..4397154 - 717 WP_001122106.1 SprT family zinc-dependent metalloprotease -
EW995_RS20975 (4397154) 4397154..4400249 - 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
EW995_RS20980 (4400268) 4400268..4401335 - 1068 WP_000634202.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
EW995_RS20985 (4401332) 4401332..4401988 - 657 WP_216349480.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
EW995_RS20990 (4402000) 4402000..4403361 - 1362 WP_241559993.1 restriction endonuclease subunit S -
EW995_RS20995 (4403361) 4403361..4405799 - 2439 WP_001392305.1 type I restriction-modification system subunit M -
EW995_RS21000 (4405839) 4405839..4406015 - 177 WP_001056892.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25216.62 Da        Isoelectric Point: 6.7630

>T119042 WP_216349480.1 NZ_CP035884:c4401988-4401332 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKRRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRAPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T119042 NZ_CP035884:c4401988-4401332 [Escherichia coli]
ATGGCCTATGACTACAGCGCCAGCCTCAACCCGCAAAAAGCCCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCGACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGGAACGTGGCAGCACACGACGTGCCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGAATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGCTCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40644.65 Da        Isoelectric Point: 5.6927

>AT119042 WP_000634202.1 NZ_CP035884:c4401335-4400268 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDAIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT119042 NZ_CP035884:c4401335-4400268 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
TGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGCGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References