Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3750799..3751417 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EW995_RS17945 Protein ID WP_001291435.1
Coordinates 3750799..3751017 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EW995_RS17950 Protein ID WP_000344800.1
Coordinates 3751043..3751417 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS17910 (3746088) 3746088..3746660 + 573 WP_000779831.1 YbaY family lipoprotein -
EW995_RS17915 (3746691) 3746691..3747002 - 312 WP_000409911.1 MGMT family protein -
EW995_RS17925 (3747381) 3747381..3747734 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EW995_RS17930 (3747776) 3747776..3749326 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EW995_RS17935 (3749490) 3749490..3749960 - 471 WP_000136192.1 YlaC family protein -
EW995_RS17940 (3750076) 3750076..3750627 - 552 WP_000102568.1 maltose O-acetyltransferase -
EW995_RS17945 (3750799) 3750799..3751017 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EW995_RS17950 (3751043) 3751043..3751417 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EW995_RS17955 (3751963) 3751963..3755112 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EW995_RS17960 (3755135) 3755135..3756328 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T119041 WP_001291435.1 NZ_CP035884:c3751017-3750799 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T119041 NZ_CP035884:c3751017-3750799 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT119041 WP_000344800.1 NZ_CP035884:c3751417-3751043 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT119041 NZ_CP035884:c3751417-3751043 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References