Detailed information of TA system TA119040

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3164525..3165357
• Replicon: chromosome
• Accession: NZ_CP035884
• Organism: Escherichia coli strain LSN03-016011/A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: EW995_RS15200
• Protein ID: WP_000854765.1
• Coordinates: 3164983..3165357 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EW995_RS15195
• Protein ID: WP_001295723.1
• Coordinates: 3164525..3164893 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW995_RS15165 (3161362)	3161362..3161817	+	456	WP_000581504.1	IrmA family protein	-
EW995_RS15170 (3161896)	3161896..3162129	+	234	WP_001119729.1	DUF905 family protein	-
EW995_RS15175 (3162229)	3162229..3163046	+	818	Protein_2959	DUF945 domain-containing protein	-
EW995_RS15180 (3163101)	3163101..3163586	+	486	WP_104895190.1	antirestriction protein	-
EW995_RS15185 (3163602)	3163602..3164078	+	477	WP_001186774.1	RadC family protein	-
EW995_RS15190 (3164141)	3164141..3164362	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EW995_RS15195 (3164525)	3164525..3164893	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EW995_RS15200 (3164983)	3164983..3165357	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
EW995_RS15205 (3165354)	3165354..3165845	+	492	WP_000976842.1	DUF5983 family protein	-
EW995_RS15210 (3165862)	3165862..3166038	+	177	WP_000839290.1	DUF957 domain-containing protein	-
EW995_RS15215 (3166144)	3166144..3166293	+	150	Protein_2967	hypothetical protein	-
EW995_RS15220 (3166948)	3166948..3169839	+	2892	WP_000580532.1	SNF2-related protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T119040 WP_000854765.1 NZ_CP035884:3164983-3165357 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T119040 NZ_CP035884:3164983-3165357 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT119040 WP_001295723.1 NZ_CP035884:3164525-3164893 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT119040 NZ_CP035884:3164525-3164893 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52