Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3065117..3065712 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EW995_RS14715 Protein ID WP_000239579.1
Coordinates 3065362..3065712 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EW995_RS14710 Protein ID WP_001223208.1
Coordinates 3065117..3065368 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS14700 (3060781) 3060781..3064560 + 3780 WP_000060921.1 autotransporter assembly complex protein TamB -
EW995_RS14705 (3064563) 3064563..3064904 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EW995_RS14710 (3065117) 3065117..3065368 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EW995_RS14715 (3065362) 3065362..3065712 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EW995_RS14720 (3065792) 3065792..3066322 - 531 WP_000055072.1 inorganic diphosphatase -
EW995_RS14725 (3066632) 3066632..3067588 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EW995_RS14730 (3067898) 3067898..3069400 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
EW995_RS14735 (3069414) 3069414..3070436 + 1023 WP_001339490.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T119039 WP_000239579.1 NZ_CP035884:3065362-3065712 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T119039 NZ_CP035884:3065362-3065712 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT119039 WP_001223208.1 NZ_CP035884:3065117-3065368 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT119039 NZ_CP035884:3065117-3065368 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References