Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2183746..2184332 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag EW995_RS10615 Protein ID WP_042014162.1
Coordinates 2184102..2184332 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EW995_RS10610 Protein ID WP_000593555.1
Coordinates 2183746..2184105 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS10585 (2179878) 2179878..2180822 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
EW995_RS10590 (2180819) 2180819..2181652 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
EW995_RS10595 (2181652) 2181652..2182416 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
EW995_RS10600 (2182413) 2182413..2183219 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
EW995_RS10605 (2183225) 2183225..2183626 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EW995_RS10610 (2183746) 2183746..2184105 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW995_RS10615 (2184102) 2184102..2184332 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
EW995_RS10620 (2184436) 2184436..2185560 - 1125 WP_001314210.1 ABC transporter permease -
EW995_RS10625 (2185560) 2185560..2188295 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T119037 WP_042014162.1 NZ_CP035884:c2184332-2184102 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T119037 NZ_CP035884:c2184332-2184102 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT119037 WP_000593555.1 NZ_CP035884:c2184105-2183746 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT119037 NZ_CP035884:c2184105-2183746 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References