Detailed information of TA system TA119029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1068290..1068915
• Replicon: chromosome
• Accession: NZ_CP035884
• Organism: Escherichia coli strain LSN03-016011/A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EW995_RS05150
• Protein ID: WP_000911330.1
• Coordinates: 1068290..1068688 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EW995_RS05155
• Protein ID: WP_000450524.1
• Coordinates: 1068688..1068915 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW995_RS05130 (1064168)	1064168..1064368	+	201	WP_000383836.1	YpfN family protein	-
EW995_RS05135 (1064478)	1064478..1065176	-	699	WP_000679812.1	esterase	-
EW995_RS05140 (1065250)	1065250..1067265	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
EW995_RS05145 (1067280)	1067280..1068143	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
EW995_RS05150 (1068290)	1068290..1068688	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
EW995_RS05155 (1068688)	1068688..1068915	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EW995_RS05160 (1069069)	1069069..1069782	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EW995_RS05165 (1069995)	1069995..1071029	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EW995_RS05170 (1071046)	1071046..1071924	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EW995_RS05175 (1072070)	1072070..1072642	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EW995_RS05180 (1072642)	1072642..1073112	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T119029 WP_000911330.1 NZ_CP035884:c1068688-1068290 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T119029 NZ_CP035884:c1068688-1068290 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT119029 WP_000450524.1 NZ_CP035884:c1068915-1068688 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT119029 NZ_CP035884:c1068915-1068688 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33