Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1068290..1068915 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EW995_RS05150 Protein ID WP_000911330.1
Coordinates 1068290..1068688 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EW995_RS05155 Protein ID WP_000450524.1
Coordinates 1068688..1068915 (-) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS05130 (1064168) 1064168..1064368 + 201 WP_000383836.1 YpfN family protein -
EW995_RS05135 (1064478) 1064478..1065176 - 699 WP_000679812.1 esterase -
EW995_RS05140 (1065250) 1065250..1067265 - 2016 WP_000829335.1 tRNA cytosine(34) acetyltransferase TmcA -
EW995_RS05145 (1067280) 1067280..1068143 - 864 WP_001267507.1 neutral zinc metallopeptidase -
EW995_RS05150 (1068290) 1068290..1068688 - 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
EW995_RS05155 (1068688) 1068688..1068915 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EW995_RS05160 (1069069) 1069069..1069782 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EW995_RS05165 (1069995) 1069995..1071029 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EW995_RS05170 (1071046) 1071046..1071924 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EW995_RS05175 (1072070) 1072070..1072642 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EW995_RS05180 (1072642) 1072642..1073112 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-125)

Antitoxin

(7-52)


Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T119029 WP_000911330.1 NZ_CP035884:c1068688-1068290 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T119029 NZ_CP035884:c1068688-1068290 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT119029 WP_000450524.1 NZ_CP035884:c1068915-1068688 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT119029 NZ_CP035884:c1068915-1068688 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References