Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 137581..138152 Replicon plasmid pWS0115A_186
Accession NZ_CP035883
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag EW996_RS24215 Protein ID WP_074502247.1
Coordinates 137925..138152 (-) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EW996_RS24210 Protein ID WP_000681610.1
Coordinates 137581..137928 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS24190 (134373) 134373..135070 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
EW996_RS24195 (135122) 135122..135730 - 609 Protein_169 DDE-type integrase/transposase/recombinase -
EW996_RS24200 (135812) 135812..136177 + 366 WP_074480516.1 transposase -
EW996_RS24205 (136177) 136177..137354 + 1178 Protein_171 IS91 family transposase -
EW996_RS24210 (137581) 137581..137928 - 348 WP_000681610.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW996_RS24215 (137925) 137925..138152 - 228 WP_074502247.1 type II toxin-antitoxin system HicA family toxin Toxin
EW996_RS24220 (138216) 138216..138893 + 678 WP_074507812.1 IS66-like element accessory protein TnpA -
EW996_RS24225 (138893) 138893..139240 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
EW996_RS24230 (139260) 139260..140831 + 1572 Protein_176 IS66 family transposase -
EW996_RS24235 (141345) 141345..141629 - 285 WP_074504064.1 hypothetical protein -
EW996_RS24240 (141651) 141651..142157 + 507 WP_074504062.1 helix-turn-helix domain-containing protein -
EW996_RS24245 (142133) 142133..142888 + 756 WP_074504059.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - csdD / csdC / csdA / csdB / estIa / etpA / etpB / eltB / eltA 1..186206 186206
- inside IScluster/Tn - eltB / eltA 134693..163214 28521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(13-74)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8668.14 Da        Isoelectric Point: 10.4156

>T119018 WP_074502247.1 NZ_CP035883:c138152-137925 [Escherichia coli]
MAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKTIKQNLKEAGVL

Download         Length: 228 bp

>T119018 NZ_CP035883:c138152-137925 [Escherichia coli]
CTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATTTTCTTCTCTAGGATATGTCAA
AAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATATTAATGCATCGCCCACATCCAG
AAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAAAGAGGCTGGAGTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13152.97 Da        Isoelectric Point: 4.9966

>AT119018 WP_000681610.1 NZ_CP035883:c137928-137581 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Download         Length: 348 bp

>AT119018 NZ_CP035883:c137928-137581 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References