Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17698..18341 Replicon plasmid pWS0115A_186
Accession NZ_CP035883
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag EW996_RS23520 Protein ID WP_077899839.1
Coordinates 17925..18341 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag EW996_RS23515 Protein ID WP_074502137.1
Coordinates 17698..17928 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS23485 (13116) 13116..13325 - 210 WP_074502145.1 HEAT repeat domain-containing protein -
EW996_RS23490 (13715) 13715..13891 + 177 WP_074502143.1 hypothetical protein -
- (14378) 14378..14429 + 52 NuclAT_3 - -
- (14378) 14378..14429 + 52 NuclAT_3 - -
- (14378) 14378..14429 + 52 NuclAT_3 - -
- (14378) 14378..14429 + 52 NuclAT_3 - -
EW996_RS23495 (14816) 14816..15067 + 252 WP_001291965.1 hypothetical protein -
EW996_RS23500 (15139) 15139..15291 - 153 WP_001303307.1 Hok/Gef family protein -
- (15341) 15341..15403 + 63 NuclAT_1 - -
- (15341) 15341..15403 + 63 NuclAT_1 - -
- (15341) 15341..15403 + 63 NuclAT_1 - -
- (15341) 15341..15403 + 63 NuclAT_1 - -
- (15644) 15644..15699 + 56 NuclAT_2 - -
- (15644) 15644..15699 + 56 NuclAT_2 - -
- (15644) 15644..15699 + 56 NuclAT_2 - -
- (15644) 15644..15699 + 56 NuclAT_2 - -
EW996_RS23505 (15893) 15893..16846 + 954 WP_021555372.1 hypothetical protein -
EW996_RS23510 (17064) 17064..17513 + 450 Protein_24 IncI1-type conjugal transfer protein TrbA -
EW996_RS23515 (17698) 17698..17928 + 231 WP_074502137.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EW996_RS23520 (17925) 17925..18341 + 417 WP_077899839.1 type II toxin-antitoxin system VapC family toxin Toxin
EW996_RS23525 (18503) 18503..20149 - 1647 WP_249531163.1 AAA family ATPase -
EW996_RS23530 (20510) 20510..20659 + 150 WP_175566211.1 hypothetical protein -
EW996_RS23535 (20659) 20659..21249 + 591 WP_074502132.1 hypothetical protein -
EW996_RS23540 (21267) 21267..21614 - 348 WP_074502130.1 DUF6404 family protein -
EW996_RS23545 (21733) 21733..22116 + 384 WP_000762576.1 colicin E1 family microcin immunity protein -
EW996_RS23550 (22064) 22064..22281 - 218 Protein_32 helix-turn-helix domain-containing protein -
EW996_RS24635 (22309) 22309..22421 - 113 Protein_33 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - csdD / csdC / csdA / csdB / estIa / etpA / etpB / eltB / eltA 1..186206 186206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15182.69 Da        Isoelectric Point: 8.7503

>T119014 WP_077899839.1 NZ_CP035883:17925-18341 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLKQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T119014 NZ_CP035883:17925-18341 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GAAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8885.01 Da        Isoelectric Point: 4.5112

>AT119014 WP_074502137.1 NZ_CP035883:17698-17928 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVRELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT119014 NZ_CP035883:17698-17928 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
AGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References