Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3723479..3723976 Replicon chromosome
Accession NZ_CP035882
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EW996_RS18140 Protein ID WP_001173343.1
Coordinates 3723479..3723652 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EW996_RS18145 Protein ID WP_000398619.1
Coordinates 3723680..3723976 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS18130 (3720144) 3720144..3721601 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EW996_RS18135 (3721838) 3721838..3723355 + 1518 WP_001295074.1 lysine--tRNA ligase -
EW996_RS18140 (3723479) 3723479..3723652 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EW996_RS18145 (3723680) 3723680..3723976 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EW996_RS18150 (3724203) 3724203..3724475 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EW996_RS18155 (3724487) 3724487..3724717 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
EW996_RS18160 (3724898) 3724898..3726529 + 1632 WP_074503002.1 sensor histidine kinase -
EW996_RS18165 (3726526) 3726526..3727245 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T119008 WP_001173343.1 NZ_CP035882:c3723652-3723479 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T119008 NZ_CP035882:c3723652-3723479 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT119008 WP_000398619.1 NZ_CP035882:c3723976-3723680 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT119008 NZ_CP035882:c3723976-3723680 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References