Detailed information of TA system TA119002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2453596..2454301
• Replicon: chromosome
• Accession: NZ_CP035882
• Organism: Escherichia coli strain WS0115A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A485DYB5
• Locus tag: EW996_RS11890
• Protein ID: WP_001743137.1
• Coordinates: 2453596..2453982 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EW996_RS11895
• Protein ID: WP_001280945.1
• Coordinates: 2453972..2454301 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW996_RS11870 (2449600)	2449600..2450226	+	627	WP_001295292.1	glutathione S-transferase GstB	-
EW996_RS11875 (2450223)	2450223..2451338	-	1116	WP_074502499.1	aldose sugar dehydrogenase YliI	-
EW996_RS11880 (2451449)	2451449..2451832	-	384	WP_000497137.1	biofilm formation regulator BssR	-
EW996_RS11885 (2452045)	2452045..2453370	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
EW996_RS11890 (2453596)	2453596..2453982	+	387	WP_001743137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EW996_RS11895 (2453972)	2453972..2454301	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
EW996_RS11900 (2454371)	2454371..2455699	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
EW996_RS11905 (2455707)	2455707..2458055	-	2349	WP_216315995.1	EAL domain-containing protein	-
EW996_RS11910 (2458233)	2458233..2459144	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.50 Da
• Isoelectric Point: 9.9296

>T119002 WP_001743137.1 NZ_CP035882:2453596-2453982 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMVNAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T119002 NZ_CP035882:2453596-2453982 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGTAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT119002 WP_001280945.1 NZ_CP035882:2453972-2454301 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT119002 NZ_CP035882:2453972-2454301 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A485DYB5