Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1831552..1832190 Replicon chromosome
Accession NZ_CP035882
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EW996_RS08820 Protein ID WP_000813794.1
Coordinates 1832014..1832190 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EW996_RS08815 Protein ID WP_001270286.1
Coordinates 1831552..1831968 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS08795 (1826704) 1826704..1827645 - 942 WP_074503096.1 ABC transporter permease -
EW996_RS08800 (1827646) 1827646..1828659 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
EW996_RS08805 (1828677) 1828677..1829822 - 1146 WP_074503097.1 ABC transporter substrate-binding protein -
EW996_RS08810 (1830067) 1830067..1831473 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
EW996_RS08815 (1831552) 1831552..1831968 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EW996_RS08820 (1832014) 1832014..1832190 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EW996_RS08825 (1832412) 1832412..1832642 + 231 WP_000494244.1 YncJ family protein -
EW996_RS08830 (1832734) 1832734..1834695 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EW996_RS08835 (1834768) 1834768..1835304 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EW996_RS08840 (1835396) 1835396..1836571 + 1176 Protein_1734 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T119001 WP_000813794.1 NZ_CP035882:c1832190-1832014 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T119001 NZ_CP035882:c1832190-1832014 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT119001 WP_001270286.1 NZ_CP035882:c1831968-1831552 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT119001 NZ_CP035882:c1831968-1831552 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCATTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References