Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 279156..279810 Replicon chromosome
Accession NZ_CP035882
Organism Escherichia coli strain WS0115A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EW996_RS01365 Protein ID WP_000244777.1
Coordinates 279403..279810 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EW996_RS01360 Protein ID WP_000354046.1
Coordinates 279156..279422 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW996_RS01335 (274325) 274325..275068 + 744 Protein_263 SDR family oxidoreductase -
EW996_RS01340 (275125) 275125..276558 - 1434 WP_139299613.1 6-phospho-beta-glucosidase BglA -
EW996_RS01345 (276603) 276603..276914 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EW996_RS01350 (277078) 277078..277737 + 660 WP_074501968.1 hemolysin III family protein -
EW996_RS01355 (277933) 277933..278913 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EW996_RS01360 (279156) 279156..279422 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EW996_RS01365 (279403) 279403..279810 + 408 WP_000244777.1 protein YgfX Toxin
EW996_RS01370 (279850) 279850..280371 - 522 WP_001055874.1 flavodoxin FldB -
EW996_RS01375 (280483) 280483..281379 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EW996_RS01380 (281404) 281404..282114 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EW996_RS01385 (282120) 282120..283853 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T118987 WP_000244777.1 NZ_CP035882:279403-279810 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118987 NZ_CP035882:279403-279810 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118987 WP_000354046.1 NZ_CP035882:279156-279422 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118987 NZ_CP035882:279156-279422 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References