Detailed information of TA system TA118983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 77353..77900
• Replicon: plasmid pDS26_81
• Accession: NZ_CP035881
• Organism: Escherichia coli strain DS26-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EW997_RS26160
• Protein ID: WP_244493824.1
• Coordinates: 77622..77900 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: EW997_RS26155
• Protein ID: WP_074573766.1
• Coordinates: 77353..77622 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW997_RS26120 (72980)	72980..73192	+	213	WP_001301409.1	ANR family transcriptional regulator	-
EW997_RS26125 (73437)	73437..73898	+	462	WP_001233850.1	thermonuclease family protein	-
EW997_RS26130 (73944)	73944..74153	+	210	WP_001301411.1	hemolysin expression modulator Hha	-
EW997_RS26135 (74191)	74191..74781	+	591	WP_000766823.1	DUF2726 domain-containing protein	-
EW997_RS26140 (75021)	75021..75281	+	261	WP_000078109.1	replication regulatory protein RepA	-
EW997_RS26145 (75516)	75516..75590	+	75	WP_001365705.1	RepA leader peptide Tap	-
EW997_RS26150 (75583)	75583..76440	+	858	WP_001388371.1	incFII family plasmid replication initiator RepA	-
EW997_RS26155 (77353)	77353..77622	+	270	WP_074573766.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
EW997_RS26160 (77622)	77622..77900	+	279	WP_244493824.1	type II toxin-antitoxin system toxin YacB	Toxin
EW997_RS26165 (77946)	77946..78170	+	225	WP_033879533.1	hypothetical protein	-
EW997_RS26170 (78171)	78171..78437	+	267	WP_000215180.1	hypothetical protein	-
EW997_RS26175 (78439)	78439..78921	+	483	WP_033879534.1	hypothetical protein	-
EW997_RS26180 (79239)	79239..79880	-	642	Protein_107	transposase	-
EW997_RS26185 (79986)	79986..80822	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
EW997_RS26190 (80822)	80822..81625	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..81772	81772
	-	flank	IS/Tn	aph(6)-Id / aph(3'')-Ib	-	79239..81625	2386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.37 Da
• Isoelectric Point: 8.4615

>T118983 WP_244493824.1 NZ_CP035881:77622-77900 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T118983 NZ_CP035881:77622-77900 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10126.47 Da
• Isoelectric Point: 10.2879

>AT118983 WP_074573766.1 NZ_CP035881:77353-77622 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLTHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT118983 NZ_CP035881:77353-77622 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTACCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA