Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 117952..118477 Replicon plasmid pDS26_129
Accession NZ_CP035880
Organism Escherichia coli strain DS26-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EW997_RS25630 Protein ID WP_001159871.1
Coordinates 117952..118257 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EW997_RS25635 Protein ID WP_000813634.1
Coordinates 118259..118477 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW997_RS25600 113208..114478 + 1271 Protein_136 translesion error-prone DNA polymerase V subunit UmuC -
EW997_RS25605 114483..114875 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
EW997_RS25610 114880..115851 - 972 WP_001103694.1 plasmid segregation protein ParM -
EW997_RS25615 116080..116724 + 645 WP_000633911.1 AAA family ATPase -
EW997_RS25620 116718..116993 + 276 WP_000239529.1 hypothetical protein -
EW997_RS25625 117131..117951 - 821 Protein_141 site-specific integrase -
EW997_RS25630 117952..118257 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EW997_RS25635 118259..118477 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EW997_RS25640 119031..121904 - 2874 WP_253207995.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - csdB / csdA / csdC / csdD / etpB / eltA / eltB 1..129918 129918


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T118982 WP_001159871.1 NZ_CP035880:c118257-117952 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T118982 NZ_CP035880:c118257-117952 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT118982 WP_000813634.1 NZ_CP035880:c118477-118259 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT118982 NZ_CP035880:c118477-118259 [Escherichia coli]
ATGAAACAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References