Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 56438..57030 Replicon plasmid pDS26_129
Accession NZ_CP035880
Organism Escherichia coli strain DS26-1

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag EW997_RS25270 Protein ID WP_074507814.1
Coordinates 56782..57030 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EW997_RS25265 Protein ID WP_000681610.1
Coordinates 56438..56785 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW997_RS25245 51966..53777 - 1812 WP_159380041.1 two-partner secretion system transporter EtpB -
EW997_RS25250 53979..54587 - 609 Protein_64 DDE-type integrase/transposase/recombinase -
EW997_RS25255 54669..55034 + 366 WP_074480516.1 transposase -
EW997_RS25260 55034..56211 + 1178 Protein_66 IS91 family transposase -
EW997_RS25265 56438..56785 - 348 WP_000681610.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW997_RS25270 56782..57030 - 249 WP_074507814.1 type II toxin-antitoxin system HicA family toxin Toxin
EW997_RS25275 57495..57779 - 285 WP_074504064.1 hypothetical protein -
EW997_RS25280 57801..58307 + 507 WP_074504062.1 helix-turn-helix domain-containing protein -
EW997_RS25285 58283..59038 + 756 WP_074504059.1 IS3 family transposase -
EW997_RS25290 59064..60277 + 1214 WP_216332910.1 IS3 family transposase -
EW997_RS25295 60313..61047 + 735 Protein_73 IS3-like element IS600 family transposase -
EW997_RS25300 61351..61785 + 435 WP_001309734.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - csdB / csdA / csdC / csdD / etpB / eltA / eltB 1..129918 129918
- inside IScluster/Tn - eltA / eltB 53979..79302 25323


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9394.03 Da        Isoelectric Point: 10.4800

>T118981 WP_074507814.1 NZ_CP035880:c57030-56782 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKTIKQNLKEAG
VL

Download         Length: 249 bp

>T118981 NZ_CP035880:c57030-56782 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATT
TTCTTCTCTAGGATATGTCAAAAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAAAGAGGCTGGA
GTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13152.97 Da        Isoelectric Point: 4.9966

>AT118981 WP_000681610.1 NZ_CP035880:c56785-56438 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Download         Length: 348 bp

>AT118981 NZ_CP035880:c56785-56438 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References