Detailed information of TA system TA118980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4444928..4445553
• Replicon: chromosome
• Accession: NZ_CP035879
• Organism: Escherichia coli strain DS26-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EW997_RS21980
• Protein ID: WP_000911330.1
• Coordinates: 4445155..4445553 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EW997_RS21975
• Protein ID: WP_000450524.1
• Coordinates: 4444928..4445155 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW997_RS21950 (4440731)	4440731..4441201	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EW997_RS21955 (4441201)	4441201..4441773	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EW997_RS21960 (4441919)	4441919..4442797	+	879	WP_097306886.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EW997_RS21965 (4442814)	4442814..4443848	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EW997_RS21970 (4444061)	4444061..4444774	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EW997_RS21975 (4444928)	4444928..4445155	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EW997_RS21980 (4445155)	4445155..4445553	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
EW997_RS21985 (4445700)	4445700..4446563	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
EW997_RS21990 (4446578)	4446578..4448593	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
EW997_RS21995 (4448667)	4448667..4449365	+	699	WP_000679823.1	esterase	-
EW997_RS22000 (4449475)	4449475..4449675	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4353194..4475404	122210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T118980 WP_000911330.1 NZ_CP035879:4445155-4445553 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118980 NZ_CP035879:4445155-4445553 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118980 WP_000450524.1 NZ_CP035879:4444928-4445155 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118980 NZ_CP035879:4444928-4445155 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33