Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4254340..4254983 Replicon chromosome
Accession NZ_CP035879
Organism Escherichia coli strain DS26-1

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag EW997_RS21040 Protein ID WP_077899839.1
Coordinates 4254340..4254756 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag EW997_RS21045 Protein ID WP_074502137.1
Coordinates 4254753..4254983 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW997_RS21015 (4249923) 4249923..4250804 + 882 WP_253207993.1 hypothetical protein -
EW997_RS21020 (4250725) 4250725..4252296 - 1572 WP_000381395.1 IS66-like element ISCro1 family transposase -
EW997_RS21025 (4252316) 4252316..4252663 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
EW997_RS21030 (4252663) 4252663..4253340 - 678 WP_074507812.1 IS66-like element accessory protein TnpA -
EW997_RS21035 (4253393) 4253393..4254178 + 786 Protein_4113 AAA family ATPase -
EW997_RS21040 (4254340) 4254340..4254756 - 417 WP_077899839.1 type II toxin-antitoxin system VapC family toxin Toxin
EW997_RS21045 (4254753) 4254753..4254983 - 231 WP_074502137.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EW997_RS21050 (4255168) 4255168..4255617 - 450 Protein_4116 IncI1-type conjugal transfer protein TrbA -
EW997_RS21055 (4255835) 4255835..4256788 - 954 WP_021555372.1 hypothetical protein -
- (4256982) 4256982..4257037 - 56 NuclAT_27 - -
- (4256982) 4256982..4257037 - 56 NuclAT_27 - -
- (4256982) 4256982..4257037 - 56 NuclAT_27 - -
- (4256982) 4256982..4257037 - 56 NuclAT_27 - -
- (4257278) 4257278..4257340 - 63 NuclAT_26 - -
- (4257278) 4257278..4257340 - 63 NuclAT_26 - -
- (4257278) 4257278..4257340 - 63 NuclAT_26 - -
- (4257278) 4257278..4257340 - 63 NuclAT_26 - -
EW997_RS21060 (4257390) 4257390..4257542 + 153 WP_001303307.1 Hok/Gef family protein -
EW997_RS21065 (4257614) 4257614..4257865 - 252 WP_001291965.1 hypothetical protein -
- (4258252) 4258252..4258303 - 52 NuclAT_28 - -
- (4258252) 4258252..4258303 - 52 NuclAT_28 - -
- (4258252) 4258252..4258303 - 52 NuclAT_28 - -
- (4258252) 4258252..4258303 - 52 NuclAT_28 - -
EW997_RS21070 (4258790) 4258790..4258966 - 177 WP_074502143.1 hypothetical protein -
EW997_RS21075 (4259356) 4259356..4259565 + 210 WP_074502145.1 HEAT repeat domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15182.69 Da        Isoelectric Point: 8.7503

>T118979 WP_077899839.1 NZ_CP035879:c4254756-4254340 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLKQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T118979 NZ_CP035879:c4254756-4254340 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GAAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8885.01 Da        Isoelectric Point: 4.5112

>AT118979 WP_074502137.1 NZ_CP035879:c4254983-4254753 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVRELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT118979 NZ_CP035879:c4254983-4254753 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
AGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References