Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3962467..3963121 Replicon chromosome
Accession NZ_CP035879
Organism Escherichia coli strain DS26-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EW997_RS19600 Protein ID WP_000244777.1
Coordinates 3962714..3963121 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EW997_RS19595 Protein ID WP_000354046.1
Coordinates 3962467..3962733 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW997_RS19570 (3957755) 3957755..3958498 + 744 WP_000951948.1 SDR family oxidoreductase -
EW997_RS19575 (3958555) 3958555..3959988 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
EW997_RS19580 (3960033) 3960033..3960344 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EW997_RS19585 (3960508) 3960508..3961167 + 660 WP_000250274.1 hemolysin III family protein -
EW997_RS19590 (3961244) 3961244..3962224 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EW997_RS19595 (3962467) 3962467..3962733 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EW997_RS19600 (3962714) 3962714..3963121 + 408 WP_000244777.1 protein YgfX Toxin
EW997_RS19605 (3963161) 3963161..3963682 - 522 WP_001055874.1 flavodoxin FldB -
EW997_RS19610 (3963794) 3963794..3964690 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EW997_RS19615 (3964715) 3964715..3965425 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EW997_RS19620 (3965431) 3965431..3967164 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T118977 WP_000244777.1 NZ_CP035879:3962714-3963121 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118977 NZ_CP035879:3962714-3963121 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118977 WP_000354046.1 NZ_CP035879:3962467-3962733 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118977 NZ_CP035879:3962467-3962733 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References