Detailed information of TA system TA118974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3369224..3369810
• Replicon: chromosome
• Accession: NZ_CP035879
• Organism: Escherichia coli strain DS26-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0H0RQZ4
• Locus tag: EW997_RS16595
• Protein ID: WP_042014162.1
• Coordinates: 3369224..3369454 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EW997_RS16600
• Protein ID: WP_086524969.1
• Coordinates: 3369451..3369810 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW997_RS16585 (3365261)	3365261..3367996	+	2736	WP_000149132.1	ribosome-associated ATPase/putative transporter RbbA	-
EW997_RS16590 (3367996)	3367996..3369120	+	1125	WP_001314210.1	ABC transporter permease	-
EW997_RS16595 (3369224)	3369224..3369454	+	231	WP_042014162.1	type II toxin-antitoxin system HicA family toxin	Toxin
EW997_RS16600 (3369451)	3369451..3369810	+	360	WP_086524969.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EW997_RS16605 (3369930)	3369930..3370331	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
EW997_RS16610 (3370337)	3370337..3371143	-	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
EW997_RS16615 (3371140)	3371140..3371904	-	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
EW997_RS16620 (3371904)	3371904..3372737	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
EW997_RS16625 (3372734)	3372734..3373678	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3350480..3369810	19330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8419.79 Da
• Isoelectric Point: 8.5518

>T118974 WP_042014162.1 NZ_CP035879:3369224-3369454 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T118974 NZ_CP035879:3369224-3369454 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13418.32 Da
• Isoelectric Point: 5.1987

>AT118974 WP_086524969.1 NZ_CP035879:3369451-3369810 [Escherichia coli]
MIKLKTLNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT118974 NZ_CP035879:3369451-3369810 [Escherichia coli]
ATGATCAAACTGAAGACGCTAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H0RQZ4