Detailed information of TA system TA118973

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2584740..2585237
• Replicon: chromosome
• Accession: NZ_CP035879
• Organism: Escherichia coli strain DS26-1

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: EW997_RS13020
• Protein ID: WP_001173343.1
• Coordinates: 2584740..2584913 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: EW997_RS13025
• Protein ID: WP_074507860.1
• Coordinates: 2584941..2585237 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW997_RS13010 (2581410)	2581410..2582867	+	1458	WP_000856841.1	dipeptide/tripeptide permease DtpC	-
EW997_RS13015 (2583104)	2583104..2584621	+	1518	WP_001295074.1	lysine--tRNA ligase	-
EW997_RS13020 (2584740)	2584740..2584913	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
EW997_RS13025 (2584941)	2584941..2585237	-	297	WP_074507860.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
EW997_RS13030 (2585464)	2585464..2585736	-	273	WP_000405647.1	GNAT family N-acetyltransferase	-
EW997_RS13035 (2585748)	2585748..2585978	-	231	WP_000371700.1	4Fe-4S mono-cluster protein YjdI	-
EW997_RS13040 (2586159)	2586159..2587790	+	1632	WP_001216487.1	sensor histidine kinase	-
EW997_RS13045 (2587787)	2587787..2588506	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T118973 WP_001173343.1 NZ_CP035879:c2584913-2584740 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T118973 NZ_CP035879:c2584913-2584740 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11493.07 Da
• Isoelectric Point: 4.2544

>AT118973 WP_074507860.1 NZ_CP035879:c2585237-2584941 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSLVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT118973 NZ_CP035879:c2585237-2584941 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTTAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41