Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2479262..2479857 Replicon chromosome
Accession NZ_CP035879
Organism Escherichia coli strain DS26-1

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EW997_RS12490 Protein ID WP_000239579.1
Coordinates 2479262..2479612 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EW997_RS12495 Protein ID WP_001223208.1
Coordinates 2479606..2479857 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW997_RS12470 (2474538) 2474538..2475560 - 1023 WP_001339490.1 ABC transporter permease -
EW997_RS12475 (2475574) 2475574..2477076 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
EW997_RS12480 (2477386) 2477386..2478342 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EW997_RS12485 (2478652) 2478652..2479182 + 531 WP_000055072.1 inorganic diphosphatase -
EW997_RS12490 (2479262) 2479262..2479612 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EW997_RS12495 (2479606) 2479606..2479857 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EW997_RS12500 (2480070) 2480070..2480411 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EW997_RS12505 (2480414) 2480414..2484193 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T118972 WP_000239579.1 NZ_CP035879:c2479612-2479262 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T118972 NZ_CP035879:c2479612-2479262 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT118972 WP_001223208.1 NZ_CP035879:c2479857-2479606 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT118972 NZ_CP035879:c2479857-2479606 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References