Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 76826..77427 Replicon plasmid pB2C_96
Accession NZ_CP035876
Organism Escherichia coli strain B2C

Toxin (Protein)


Gene name doc Uniprot ID A0A6G4LZ59
Locus tag EW998_RS24805 Protein ID WP_001216040.1
Coordinates 77047..77427 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID I2USI4
Locus tag EW998_RS24800 Protein ID WP_001190710.1
Coordinates 76826..77047 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW998_RS24745 72210..72701 + 492 WP_001167293.1 hypothetical protein -
EW998_RS24750 72703..72966 + 264 WP_000224221.1 hypothetical protein -
EW998_RS26080 72977..73102 + 126 Protein_88 hypothetical protein -
EW998_RS24755 73076..73663 + 588 WP_248841495.1 DUF551 domain-containing protein -
EW998_RS24760 73663..73923 + 261 WP_001554875.1 hypothetical protein -
EW998_RS24765 73923..74204 + 282 WP_032323268.1 ASCH domain-containing protein -
EW998_RS24770 74228..74521 + 294 WP_032323267.1 hypothetical protein -
EW998_RS24775 74546..74755 + 210 WP_032323266.1 hypothetical protein -
EW998_RS24780 74752..75549 + 798 WP_064736190.1 hypothetical protein -
EW998_RS26085 75606..75956 - 351 WP_032323265.1 hypothetical protein -
EW998_RS24790 75989..76240 + 252 WP_032323264.1 DNA polymerase III subunit theta -
EW998_RS24795 76364..76753 + 390 WP_000506724.1 S24 family peptidase -
EW998_RS24800 76826..77047 + 222 WP_001190710.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
EW998_RS24805 77047..77427 + 381 WP_001216040.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EW998_RS24810 77463..78557 - 1095 WP_032323263.1 hypothetical protein -
EW998_RS24815 78547..79314 - 768 WP_032323262.1 hypothetical protein -
EW998_RS24820 79321..79983 - 663 WP_032152851.1 DUF2829 domain-containing protein -
EW998_RS24825 79998..80492 - 495 WP_032323258.1 dUTP diphosphatase -
EW998_RS24830 80489..80965 - 477 WP_032323256.1 hypothetical protein -
EW998_RS24835 80962..81270 - 309 WP_032323254.1 hypothetical protein -
EW998_RS24840 81380..82408 - 1029 WP_032323253.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..96149 96149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13678.59 Da        Isoelectric Point: 6.2282

>T118961 WP_001216040.1 NZ_CP035876:77047-77427 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDDPVLVELAVGAATGEIPVSSVAKKLRELYGSNI

Download         Length: 381 bp

>T118961 NZ_CP035876:77047-77427 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATGATCCCGTGCTGGTGGAGCTTGCAGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGAAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT118961 WP_001190710.1 NZ_CP035876:76826-77047 [Escherichia coli]
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT118961 NZ_CP035876:76826-77047 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAATTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAGGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4LZ59

Antitoxin

Source ID Structure
AlphaFold DB A0A348ERB2

References