Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90442..90967 Replicon plasmid pB2C_151
Accession NZ_CP035875
Organism Escherichia coli strain B2C

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EW998_RS23940 Protein ID WP_001159871.1
Coordinates 90662..90967 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EW998_RS23935 Protein ID WP_000813634.1
Coordinates 90442..90660 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW998_RS23910 (86078) 86078..87028 - 951 WP_001699589.1 virulence factor VirK -
EW998_RS23915 (87033) 87033..88121 - 1089 WP_004100285.1 putative hexosyltransferase CapU -
EW998_RS23920 (88124) 88124..88966 - 843 WP_160371899.1 polysaccharide deacetylase family protein -
EW998_RS23925 (89210) 89210..89740 - 531 WP_011387704.1 hypothetical protein -
EW998_RS23930 (89740) 89740..89997 - 258 WP_000343092.1 hypothetical protein -
EW998_RS23935 (90442) 90442..90660 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EW998_RS23940 (90662) 90662..90967 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EW998_RS23945 (90992) 90992..91681 + 690 Protein_110 site-specific integrase -
EW998_RS23950 (92011) 92011..92652 + 642 WP_000470631.1 recombinase family protein -
EW998_RS23955 (93109) 93109..93771 - 663 WP_032323101.1 EAL domain-containing protein -
EW998_RS23960 (94019) 94019..94723 + 705 WP_001119661.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpB / cs3 / eltA / eltB 1..151583 151583
- flank IS/Tn - - 92011..92652 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T118957 WP_001159871.1 NZ_CP035875:90662-90967 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T118957 NZ_CP035875:90662-90967 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT118957 WP_000813634.1 NZ_CP035875:90442-90660 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT118957 NZ_CP035875:90442-90660 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References