Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4719509..4720006 Replicon chromosome
Accession NZ_CP035874
Organism Escherichia coli strain B2C

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EW998_RS22910 Protein ID WP_001173343.1
Coordinates 4719509..4719682 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag EW998_RS22915 Protein ID WP_071597404.1
Coordinates 4719710..4720006 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW998_RS22900 (4716174) 4716174..4717631 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EW998_RS22905 (4717868) 4717868..4719385 + 1518 WP_001295074.1 lysine--tRNA ligase -
EW998_RS22910 (4719509) 4719509..4719682 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EW998_RS22915 (4719710) 4719710..4720006 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EW998_RS22920 (4720233) 4720233..4720505 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EW998_RS22925 (4720517) 4720517..4720747 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
EW998_RS22930 (4720928) 4720928..4722559 + 1632 WP_001216490.1 sensor histidine kinase -
EW998_RS22935 (4722556) 4722556..4723275 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118956 WP_001173343.1 NZ_CP035874:c4719682-4719509 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118956 NZ_CP035874:c4719682-4719509 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT118956 WP_071597404.1 NZ_CP035874:c4720006-4719710 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118956 NZ_CP035874:c4720006-4719710 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References