Detailed information of TA system TA118951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2843349..2843987
• Replicon: chromosome
• Accession: NZ_CP035874
• Organism: Escherichia coli strain B2C

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EW998_RS13665
• Protein ID: WP_000813794.1
• Coordinates: 2843811..2843987 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EW998_RS13660
• Protein ID: WP_001270286.1
• Coordinates: 2843349..2843765 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW998_RS13640 (2838501)	2838501..2839442	-	942	WP_001251313.1	ABC transporter permease	-
EW998_RS13645 (2839443)	2839443..2840456	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
EW998_RS13650 (2840474)	2840474..2841619	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
EW998_RS13655 (2841864)	2841864..2843270	-	1407	WP_000760586.1	PLP-dependent aminotransferase family protein	-
EW998_RS13660 (2843349)	2843349..2843765	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EW998_RS13665 (2843811)	2843811..2843987	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EW998_RS13670 (2844209)	2844209..2844439	+	231	WP_000494244.1	YncJ family protein	-
EW998_RS13675 (2844531)	2844531..2846492	-	1962	WP_001355676.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EW998_RS13680 (2846565)	2846565..2847101	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
EW998_RS13685 (2847193)	2847193..2848368	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2848408..2849673	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T118951 WP_000813794.1 NZ_CP035874:c2843987-2843811 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T118951 NZ_CP035874:c2843987-2843811 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT118951 WP_001270286.1 NZ_CP035874:c2843765-2843349 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT118951 NZ_CP035874:c2843765-2843349 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB