Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1290015..1290669 Replicon chromosome
Accession NZ_CP035874
Organism Escherichia coli strain B2C

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EW998_RS06265 Protein ID WP_000244781.1
Coordinates 1290262..1290669 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EW998_RS06260 Protein ID WP_000354046.1
Coordinates 1290015..1290281 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW998_RS06240 (1286103) 1286103..1287536 - 1434 WP_032323017.1 6-phospho-beta-glucosidase BglA -
EW998_RS06245 (1287581) 1287581..1287892 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EW998_RS06250 (1288056) 1288056..1288715 + 660 WP_000250274.1 hemolysin III family protein -
EW998_RS06255 (1288792) 1288792..1289772 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
EW998_RS06260 (1290015) 1290015..1290281 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EW998_RS06265 (1290262) 1290262..1290669 + 408 WP_000244781.1 protein YgfX Toxin
EW998_RS06270 (1290709) 1290709..1291230 - 522 WP_001055874.1 flavodoxin FldB -
EW998_RS06275 (1291342) 1291342..1292238 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
EW998_RS06280 (1292263) 1292263..1292973 + 711 WP_000715224.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EW998_RS06285 (1292979) 1292979..1294712 + 1734 WP_001394741.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118940 WP_000244781.1 NZ_CP035874:1290262-1290669 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118940 NZ_CP035874:1290262-1290669 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118940 WP_000354046.1 NZ_CP035874:1290015-1290281 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118940 NZ_CP035874:1290015-1290281 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References