Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RelE-RHH |
| Location | 34935..35482 | Replicon | plasmid pE2528C1_53 |
| Accession | NZ_CP035873 | ||
| Organism | Escherichia coli strain E2528-C1 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | G7QEJ9 |
| Locus tag | EW999_RS25335 | Protein ID | WP_001384452.1 |
| Coordinates | 35204..35482 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | Q4FIL6 |
| Locus tag | EW999_RS25330 | Protein ID | WP_000079945.1 |
| Coordinates | 34935..35204 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EW999_RS25290 (30008) | 30008..30355 | + | 348 | WP_000624622.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EW999_RS25295 (30375) | 30375..31946 | + | 1572 | WP_000381395.1 | IS66-like element ISCro1 family transposase | - |
| EW999_RS25300 (31987) | 31987..32136 | + | 150 | WP_077637090.1 | traC domain protein | - |
| EW999_RS25310 (32437) | 32437..32697 | + | 261 | WP_005768671.1 | replication regulatory protein RepA | - |
| EW999_RS25755 (32723) | 32723..32962 | - | 240 | WP_235838394.1 | replication protein RepA | - |
| EW999_RS25760 (32953) | 32953..33000 | + | 48 | WP_235838393.1 | hypothetical protein | - |
| EW999_RS25320 (32993) | 32993..34015 | + | 1023 | WP_032200411.1 | plasmid replication initiator RepA | - |
| EW999_RS25325 (34722) | 34722..34862 | + | 141 | WP_166426637.1 | hypothetical protein | - |
| EW999_RS25330 (34935) | 34935..35204 | + | 270 | WP_000079945.1 | type II toxin-antitoxin system antitoxin YacA | Antitoxin |
| EW999_RS25335 (35204) | 35204..35482 | + | 279 | WP_001384452.1 | type II toxin-antitoxin system toxin YacB | Toxin |
| EW999_RS25340 (35554) | 35554..35732 | + | 179 | Protein_48 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EW999_RS25345 (35803) | 35803..36684 | + | 882 | WP_000005461.1 | hypothetical protein | - |
| EW999_RS25350 (36619) | 36619..37038 | - | 420 | WP_024181414.1 | hypothetical protein | - |
| EW999_RS25355 (37147) | 37147..37497 | - | 351 | WP_001414994.1 | plasmid partitioning/stability family protein | - |
| EW999_RS25360 (37500) | 37500..38462 | - | 963 | WP_000959884.1 | plasmid segregation protein ParM | - |
| EW999_RS25765 (38609) | 38609..38902 | + | 294 | WP_047081703.1 | hypothetical protein | - |
| EW999_RS25365 (38997) | 38997..39899 | + | 903 | WP_216311848.1 | DUF1281 domain-containing protein | - |
| EW999_RS25370 (39896) | 39896..40207 | + | 312 | WP_167553962.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | cofA | 1..53738 | 53738 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10762.38 Da Isoelectric Point: 8.4615
>T118935 WP_001384452.1 NZ_CP035873:35204-35482 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
Download Length: 279 bp
>T118935 NZ_CP035873:35204-35482 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10180.52 Da Isoelectric Point: 8.8735
>AT118935 WP_000079945.1 NZ_CP035873:34935-35204 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
Download Length: 270 bp
>AT118935 NZ_CP035873:34935-35204 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTTCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTTCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CL36 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q4FIL6 |