Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 4746..5305 Replicon plasmid pE2528C1_96
Accession NZ_CP035871
Organism Escherichia coli strain E2528-C1

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag EW999_RS24080 Protein ID WP_000421257.1
Coordinates 4746..5021 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag EW999_RS24085 Protein ID WP_001178089.1
Coordinates 5021..5305 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW999_RS24055 672..1058 - 387 WP_000460651.1 hypothetical protein -
EW999_RS24060 1051..1371 - 321 WP_000562370.1 antibiotic biosynthesis monooxygenase -
EW999_RS24065 1815..3020 + 1206 WP_000599533.1 sodium/glutamate symporter -
EW999_RS24070 3251..3370 + 120 Protein_4 LysR family transcriptional regulator -
EW999_RS24075 3386..4594 - 1209 WP_001352368.1 IS4-like element ISVsa5 family transposase -
EW999_RS24080 4746..5021 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EW999_RS24085 5021..5305 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
EW999_RS24090 6210..7082 - 873 WP_000729438.1 incFII family plasmid replication initiator RepA -
EW999_RS24095 7786..8427 + 642 WP_000335849.1 transcription termination/antitermination NusG family protein -
EW999_RS24100 8568..9230 + 663 WP_000154142.1 hypothetical protein -
EW999_RS24105 9497..10117 + 621 WP_000176497.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / tet(B) - 1..95804 95804
- flank IS/Tn - - 3386..4594 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T118929 WP_000421257.1 NZ_CP035871:c5021-4746 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T118929 NZ_CP035871:c5021-4746 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT118929 WP_001178089.1 NZ_CP035871:c5305-5021 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT118929 NZ_CP035871:c5305-5021 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References