Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4182634..4183288 Replicon chromosome
Accession NZ_CP035870
Organism Escherichia coli strain E2528-C1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EW999_RS20540 Protein ID WP_000244781.1
Coordinates 4182881..4183288 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EW999_RS20535 Protein ID WP_000354046.1
Coordinates 4182634..4182900 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW999_RS20515 (4178722) 4178722..4180155 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
EW999_RS20520 (4180200) 4180200..4180511 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EW999_RS20525 (4180675) 4180675..4181334 + 660 WP_000250274.1 hemolysin III family protein -
EW999_RS20530 (4181411) 4181411..4182391 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
EW999_RS20535 (4182634) 4182634..4182900 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EW999_RS20540 (4182881) 4182881..4183288 + 408 WP_000244781.1 protein YgfX Toxin
EW999_RS20545 (4183328) 4183328..4183849 - 522 WP_001055874.1 flavodoxin FldB -
EW999_RS20550 (4183961) 4183961..4184857 + 897 WP_001735667.1 site-specific tyrosine recombinase XerD -
EW999_RS20555 (4184882) 4184882..4185592 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EW999_RS20560 (4185598) 4185598..4187331 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118927 WP_000244781.1 NZ_CP035870:4182881-4183288 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118927 NZ_CP035870:4182881-4183288 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118927 WP_000354046.1 NZ_CP035870:4182634-4182900 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118927 NZ_CP035870:4182634-4182900 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References