Detailed information of TA system TA118926

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4088340..4089172
• Replicon: chromosome
• Accession: NZ_CP035870
• Organism: Escherichia coli strain E2528-C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: EW999_RS20040
• Protein ID: WP_000854765.1
• Coordinates: 4088340..4088714 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EW999_RS20045
• Protein ID: WP_001295723.1
• Coordinates: 4088804..4089172 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW999_RS20020 (4086132)	4086132..4087352	+	1221	WP_001735713.1	capsular biosynthesis protein	-
EW999_RS20025 (4087378)	4087378..4087608	-	231	Protein_3924	hypothetical protein	-
EW999_RS20030 (4087714)	4087714..4087890	-	177	WP_000839288.1	DUF957 domain-containing protein	-
EW999_RS20035 (4087907)	4087907..4088343	-	437	Protein_3926	DUF5983 family protein	-
EW999_RS20040 (4088340)	4088340..4088714	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
EW999_RS20045 (4088804)	4088804..4089172	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EW999_RS20050 (4089335)	4089335..4089556	-	222	WP_001735710.1	DUF987 domain-containing protein	-
EW999_RS20055 (4089625)	4089625..4090101	-	477	WP_001186725.1	RadC family protein	-
EW999_RS20060 (4090117)	4090117..4090602	-	486	WP_001735709.1	antirestriction protein	-
EW999_RS20065 (4090657)	4090657..4091475	-	819	WP_074481115.1	DUF932 domain-containing protein	-
EW999_RS20070 (4091593)	4091593..4091788	-	196	Protein_3933	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T118926 WP_000854765.1 NZ_CP035870:c4088714-4088340 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T118926 NZ_CP035870:c4088714-4088340 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT118926 WP_001295723.1 NZ_CP035870:c4089172-4088804 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118926 NZ_CP035870:c4089172-4088804 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52