Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2572983..2573559 Replicon chromosome
Accession NZ_CP035870
Organism Escherichia coli strain E2528-C1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EW999_RS12820 Protein ID WP_001297643.1
Coordinates 2573272..2573559 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EW999_RS12815 Protein ID WP_000063156.1
Coordinates 2572983..2573285 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW999_RS12800 (2569579) 2569579..2571729 + 2151 WP_001736090.1 pyruvate/proton symporter BtsT -
EW999_RS12805 (2571779) 2571779..2571982 + 204 WP_000467859.1 YbdD/YjiX family protein -
EW999_RS12810 (2571993) 2571993..2572949 + 957 WP_001297640.1 GTPase -
EW999_RS12815 (2572983) 2572983..2573285 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EW999_RS12820 (2573272) 2573272..2573559 - 288 WP_001297643.1 BrnT family toxin Toxin
EW999_RS12825 (2573758) 2573758..2574455 - 698 Protein_2518 IS1 family transposase -
EW999_RS12830 (2574512) 2574512..2574607 + 96 Protein_2519 dienelactone hydrolase family protein -
EW999_RS12835 (2574753) 2574753..2575985 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EW999_RS12840 (2576026) 2576026..2577306 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T118923 WP_001297643.1 NZ_CP035870:c2573559-2573272 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T118923 NZ_CP035870:c2573559-2573272 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT118923 WP_000063156.1 NZ_CP035870:c2573285-2572983 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT118923 NZ_CP035870:c2573285-2572983 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References