Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1994186..1994804 Replicon chromosome
Accession NZ_CP035870
Organism Escherichia coli strain E2528-C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EW999_RS10025 Protein ID WP_001291435.1
Coordinates 1994586..1994804 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EW999_RS10020 Protein ID WP_000344800.1
Coordinates 1994186..1994560 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW999_RS10010 (1989275) 1989275..1990468 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EW999_RS10015 (1990491) 1990491..1993640 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EW999_RS10020 (1994186) 1994186..1994560 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EW999_RS10025 (1994586) 1994586..1994804 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EW999_RS10030 (1994976) 1994976..1995527 + 552 WP_001727569.1 maltose O-acetyltransferase -
EW999_RS10035 (1995643) 1995643..1996113 + 471 WP_000136192.1 YlaC family protein -
EW999_RS10040 (1996277) 1996277..1997827 + 1551 WP_001727568.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EW999_RS10045 (1997869) 1997869..1998222 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EW999_RS10055 (1998601) 1998601..1998912 + 312 WP_000409911.1 MGMT family protein -
EW999_RS10060 (1998943) 1998943..1999515 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118920 WP_001291435.1 NZ_CP035870:1994586-1994804 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118920 NZ_CP035870:1994586-1994804 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118920 WP_000344800.1 NZ_CP035870:1994186-1994560 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118920 NZ_CP035870:1994186-1994560 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References