Detailed information of TA system TA118908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 9168..9715
• Replicon: plasmid pE275a_90
• Accession: NZ_CP035868
• Organism: Escherichia coli strain E275 a

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: EXE34_RS25945
• Protein ID: WP_001384452.1
• Coordinates: 9437..9715 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: EXE34_RS25940
• Protein ID: WP_000079928.1
• Coordinates: 9168..9437 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE34_RS25910 (4604)	4604..4813	+	210	WP_088568712.1	hypothetical protein	-
EXE34_RS25915 (5225)	5225..5851	+	627	WP_088568713.1	NYN domain-containing protein	-
EXE34_RS25920 (6003)	6003..6593	+	591	WP_044311467.1	DUF2726 domain-containing protein	-
EXE34_RS25925 (6833)	6833..7081	+	249	WP_000083839.1	replication regulatory protein RepA	-
EXE34_RS25930 (7328)	7328..7402	+	75	WP_001365705.1	RepA leader peptide Tap	-
EXE34_RS25935 (7395)	7395..8252	+	858	WP_000131014.1	incFII family plasmid replication initiator RepA	-
EXE34_RS25940 (9168)	9168..9437	+	270	WP_000079928.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
EXE34_RS25945 (9437)	9437..9715	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
EXE34_RS25950 (9761)	9761..10609	+	849	WP_042815954.1	3'-5' exonuclease	-
EXE34_RS25955 (10750)	10750..11208	+	459	WP_253207950.1	hypothetical protein	-
EXE34_RS25960 (11434)	11434..12456	+	1023	WP_159387386.1	IS21-like element IS100 family transposase	-
EXE34_RS25965 (12453)	12453..13235	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EXE34_RS25970 (13464)	13464..13640	+	177	Protein_18	transposase domain-containing protein	-
EXE34_RS26790 (13674)	13674..13828	-	155	Protein_19	transposase	-
EXE34_RS25980 (13823)	13823..14074	-	252	Protein_20	IS66 family insertion sequence element accessory protein TnpB	-
EXE34_RS26795 (14071)	14071..14229	-	159	WP_235835353.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / eltA / eltB / cswA	1..90662	90662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T118908 WP_001384452.1 NZ_CP035868:9437-9715 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T118908 NZ_CP035868:9437-9715 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT118908 WP_000079928.1 NZ_CP035868:9168-9437 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT118908 NZ_CP035868:9168-9437 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A5W2J4D0