Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 46853..47378 Replicon plasmid pE275a_100
Accession NZ_CP035867
Organism Escherichia coli strain E275 a

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EXE34_RS25565 Protein ID WP_001159871.1
Coordinates 46853..47158 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EXE34_RS25570 Protein ID WP_000813634.1
Coordinates 47160..47378 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE34_RS25545 (43096) 43096..43800 - 705 WP_134692445.1 helix-turn-helix domain-containing protein -
EXE34_RS25550 (44048) 44048..44728 + 681 WP_000452091.1 EAL domain-containing protein -
EXE34_RS25555 (45168) 45168..45809 - 642 WP_000470631.1 recombinase family protein -
EXE34_RS25560 (46139) 46139..46828 - 690 Protein_52 site-specific integrase -
EXE34_RS25565 (46853) 46853..47158 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EXE34_RS25570 (47160) 47160..47378 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EXE34_RS25575 (47823) 47823..48080 + 258 WP_000343092.1 hypothetical protein -
EXE34_RS25580 (48080) 48080..48610 + 531 WP_011387704.1 hypothetical protein -
EXE34_RS25585 (48854) 48854..49696 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
EXE34_RS25590 (49699) 49699..50787 + 1089 WP_001388979.1 putative hexosyltransferase CapU -
EXE34_RS25595 (50792) 50792..51742 + 951 WP_001699589.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpA / etpB 1..100141 100141
- flank IS/Tn - - 45168..45809 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T118904 WP_001159871.1 NZ_CP035867:c47158-46853 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T118904 NZ_CP035867:c47158-46853 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT118904 WP_000813634.1 NZ_CP035867:c47378-47160 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT118904 NZ_CP035867:c47378-47160 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References